|
KMID : 1161520060100020073
|
|
Animal Cells and Systems
2006 Volume.10 No. 2 p.73 ~ p.77
|
|
|
Caspase-11 promoter-GFP construct as a dual reporter of cytotoxicity and inflammation
|
|
Shin Ki-Soon
Kang Shin-Jung
|
|
|
Abstract
|
|
|
|
Caspase?11 has been known as a dual regulator of apoptosis and inflammatory response. An unusual feature of caspase?11 is that its expression is induced by apoptotic or proinflammatory stimuli. Utilizing these unusual features of caspase?11, we have developed a simple and sensitive assay method to screen pro? or anti?apoptotic/inflammatory molecules. To develop this assay method, we generated a reporter construct where GFP expression is regulated by caspase?11 promoter. When several types of cultured cells were transfected with this reporter construct and subsequently treated with various apoptotic or proinflammatory molecules, expression of GFP by the activation of caspase?11 promoter was easily detected by fluorescence microscopy or spectro?fluorometry. In addition, a reduction of the GFP fluorescence was detected when an agent reported to suppress caspase?11 induction was applied. These results suggest that our reporter system can be used to screen pro? or anti?apoptotic/ inflammatory molecules.
|
|
|
KEYWORD
|
|
|
Caspase-11 promoter-GFP, cytotoxicity, inflammation
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|